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61.
J. G. Dubouzet K. Shinoda 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):213-218
Cycle sequencing of the ITS region of nuclear ribosomal DNA of L. concolor, L. dauricum and L. maculatum generated surprisingly homogenous sequences from these three species. Analysis of the few (13 out of 639) polymorphic nucleotide
sites in the ITS region produced results that do not support the belief that L. maculatum is a hybrid of the two other species. Neighbor-Joining analysis of the genetic distances calculated using the Kimura 2-parameter
model of base substitution confirmed the close relationship between L. dauricum and L. maculatum. The phylogenetic tree, in conjunction with the distribution pattern and morphological similarities of the two species, suggest
that L. maculatum is derived from the more widely distributed L. dauricum. The results also revealed that there is sufficient molecular divergence between L. maculatum and L. dauricum to support their status as separate species.
Received: 4 June 1998 / Accepted: 15 July 1998 相似文献
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Homologues of glucosephosphate isomerase (GPI, EC 5.3.1.9) were purified to
homogeneity and kinetically characterized from Mytilus edulis and Isognomon
alatus, two bivalve molluscs experiencing contrasting thermal environments.
The enzyme isolated from I. alatus functions at warmer temperatures (25-35
C) than GPI from M. edulis, a species that inhabits colder marine littoral
habitats (5-20 C). The former exhibits apparent first-order (with respect
to substrate) catalytic rate constants (Vmax/KM) in vitro that become
progressively greater than the mussel enzyme as the assay temperature is
raised. Apparent zero-order catalytic rate constants (Vmax) are relatively
less differentiated. Catalytic efficiency, defined as the rate at which a
catalytic event occurs in either reaction direction for reference standard
states (substrate concentrations), is greater for the enzyme from the
tropical species (I. alatus) at all realistic combinations of temperature
and substrate concentration except for the lowest temperatures and highest
substrate concentrations, where the GPI from the boreal/temperate M. edulis
is more efficient. This pattern of catalytic divergence appears to be due
primarily to differentiation in Vmax/KM. These results and other published
data are reviewed and shown to be inconsistent with claims that adaptation
of enzymes to higher cell temperatures requires a loss in catalytic
efficiency.
相似文献
65.
Ryoji Takahashi Joseph G Dubouzet Hisakazu Matsumura Kentaro Yasuda Tsukasa Iwashina 《BMC plant biology》2010,10(1):155
Background
Glycine soja is a wild relative of soybean that has purple flowers. No flower color variant of Glycine soja has been found in the natural habitat. 相似文献66.
Marlies van Nimwegen Arlène D Speelman Katrijn Smulders Sebastiaan Overeem George F Borm Frank JG Backx Bastiaan R Bloem Marten Munneke ParkFit study group 《BMC neurology》2010,10(1):70
Background
Many patients with Parkinson's disease (PD) lead a sedentary lifestyle. Promotion of physical activities may beneficially affect the clinical presentation of PD, and perhaps even modify the course of PD. However, because of physical and cognitive impairments, patients with PD require specific support to increase their level of physical activity. 相似文献67.
M Kale R Ramsey-Goldman S Bernatsky MB Urowitz D Gladman PR Fortin M Petri E Yelin S Manzi S Edworthy O Nived S-C Bae D Isenberg A Rahman JG Hanly C Gordon S Jacobsen E Ginzler DJ Wallace GS Alarcón MA Dooley L Gottesman K Steinsson A Zoma J-L Senécal S Barr G Sturfelt L Dreyer L Criswell J Sibley JL Lee AE Clarke 《Arthritis research & therapy》2012,14(Z3):A15
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